Sanitary and epidemiological supervision of population nutrition should ensure protection of the population from the harmful effects of contaminants entering the body with food products. Aflatoxin B1 is one of the most common and toxic mycotoxicants of food, increasing the risks of developing various diseases, including malignant neoplasms. The purpose of the study is a hygienic assessment of the risk load on the health of various professional groups of the working-age population in terms of aflatoxin B1 intake with food products. Materials and methods. An assessment of the actual nutrition of various professional groups of the working-age population of the Samara region (groups "industry", "education", "medicine", office workers", "agriculture") was carried out. A total of 1943 people (1078 women and 865 men) aged 18 to 65 years took part in the study. Data on the average annual consumption of a group of products were obtained automatically using the Nutri-prof software product (version 2.9). The study of aflatoxin B1 content in food products was carried out according to the data of the laboratory of the Federal Budgetary Institution of Health "Center for Hygiene and Epidemiology in the Samara Region" based on the methods used - HPLC and TLC. The total number of analyzed food samples was 1542. Health risk assessment by the hazard coefficient was carried out taking into account the median values and 90 percentile of aflatoxin content in the following groups of food products (vegetable oil and other fats, mayonnaise; bread and bread products, cereals, oatmeal, flakes, nuts; sugar and confectionery (including cocoa beans and cocoa products)). The results and their discussion. The highest values of aflatoxin B1 concentration were found in vegetable oils and other fats, mayonnaise and were due to the use of sunflower seeds in the manufacturing process of products containing high values of the contaminant (Me - 0.0002 mg/kg, 90 percentile - 0.005 mg/kg). The main contribution to mycotoxicant exposure was made by such food groups as bread and bread products, cereals, oatmeal, flakes, nuts; vegetable oil and other fats (unrefined oil, mayonnaise), sugar and confectionery (cocoa beans, chocolate). The highest health risk, obtained on the basis of calculating the hazard coefficient taking into account the actual consumption of food products, was noted in the professional group "agriculture" in women - 0.028 (Me) and 0.35 (90 percentile). Among men in the “agriculture” group - 0.023 (Me) and “industry” - 0.023 (Me), under the scenario of maximum contaminant intake (90th percentile) among men in the “industry” group - 0.26 and “education” - 0.26. Conclusion. The levels of health risks in various professional groups of the working population as a result of the intake of aflatoxin B1 under various intake scenarios corresponded to the permissible range, which indicates the effective work of the state sanitary and epidemiological supervision authorities to prevent the circulation of food products that pose a danger to the health of consumers. Persons who have professional contact with production factors that negatively affect liver function, as well as with chronic liver diseases, need to take into account the intake of aflatoxin B1 with potentially dangerous food products, reducing their intake, and increasing the adaptive potential of the body.